Aim: To lay down the Standard Operating Procedure (SOP) for Operating Laminar Airflow in a Microbiology Laboratory.
Scope: This SOP applies to carry out the procedure for Operating Laminar Airflow in a Microbiology Laboratory.
Safety precautions:
a. Wear appropriate personal protective equipment (PPE), including a lab coat, gloves, and safety glasses.
b. Do not obstruct the airflow by placing objects on top of the laminar airflow unit or in the path of the air.
c. Keep the laboratory area clean and organized to prevent contamination.
Preparing the laminar airflow unit:
a. Turn on the unit at least 15 minutes before use to allow it to reach its operating temperature.
b. Clean the surfaces of the unit with a suitable disinfectant.
c. Verify that the unit’s HEPA filter is clean and in good condition.
Related: SOP for Operation and cleaning of Vernier Caliper
Using the laminar airflow unit:
a. Place all necessary materials and equipment inside the laminar airflow unit before turning it on.
b. Position the materials and equipment to be used in the center of the unit to ensure proper airflow.
c. Do not disturb the laminar airflow by reaching into the unit or by placing objects on top of it while it is in use.
d. Work carefully and avoid sudden movements to minimize disruption of the laminar airflow.
e. Close the sash or front cover of the unit when not in use to prevent contamination.
Cleaning and maintenance:
a. Clean the surfaces of the unit with a suitable disinfectant after each use.
b. Replace the HEPA filter according to the manufacturer’s instructions or laboratory policy.
c. Verify that the laminar airflow unit is functioning properly before each use.
ABBREVIATION DEFINITION:
QA- Quality assurance
QC- Quality Control
SOP- Standard operating procedure
LAF– Laminar airflow
UV- Ultraviolet
GDP– good document practices